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澳门老虎机 > 新闻资讯 > 制备微检测培养基细节问题
制备微检测培养基细节问题
发布时间:2018/7/16 11:38:17   点击次数:1835

YI、CHENGQU

澳门老虎机YONGDU1/100DEDIANZITIANPINGCHENGQUPEIYANGJISUOXU。XIANGENJUPEIFANGJISUANCHUPEIZHIYIDINGLIANGPEIYANGJISUOXUGEZHONGCHENGFENDELIANG,RANHOUYONGTIANPINGZHUNQUECHENGQU。CHENGLIANGSHIYONGCHENGLIANGZHIZHEDIECHENGBOJIZHUANGSHENGFANG。CHENGLIANGZHIZHEDIEFANGFARUTUSUOSHI。

ER、RONGHUA

PEIYANGJIFANGRUSHAOBEIHUOTANGCIGANGZHONG,MANMANJIARUSHAOLIANGSUOXUSHUI,BIANJIARUBIANYONGBOBANGJIAOBAN。RUGUOPEIYANGJIZHONGBUHANYOUQIONGZHI,PEIYANGJIBUXUYAOJIARE;RUGUOHANYOUQIONGZHI,ZEXUYAOYONGBENSHENGDENGHUODIANCILUJIAREZHUFEI,WANQUANRONGJIEHOU,ZAIBUQISUOXUSHUI,BINGJIAOBANJUNYUN(RUTU3SUOSHI)。RUGUOPEIZHIDEPEIYANGJILIANGHENDA,KEYONGBUXIUGANGGUOJIARERONGHUA,KEXIANYONGWENSHUIJIAREBINGSUISHIJIAODONG,FANGZHIJIAOHUA,RUYOUJIAOHUAXIANXIANG,PEIZHIDEPEIYANGJIJIUBUNENGSHIYONG,YAOZHONGXINPEIZHI。

 

PEIZHIPEIYANGJISHIBUKEYONGTONGHUOTIEDERONGQIRONGHUA,YINTONGHUOTIEZHIRONGQIKENENGHUISHIPEIYANGJIZHONGDETONGHETIEDEHANLIANGCHAOBIAO,YINGXIANGSHIYAN(PEIYANGJIZHONGTONGHANLIANGDAYU0.3mg/L,XIJUNBUYISHENGZHANG,TIEHANLIANGCHAOGUO0.14mg/L,FANGAIXIJUNCHANDUSU)。DUIRONGYIFASHENGFANYING,CHANSHENGCHENDIANDE,YAOFENKAIRONGJIE,HOUJIARUPEIYANGJI,RULINSUANQINGERJIAHELIUSUANMEI。

SAN、DIAOpH

澳门老虎机SUIRANPEIYANGJIZHONGHANYOUHUANCHONGWUZHICHENGFEN,NENGSHIPEIYANGJIDEpHJINKENENGDEBAOCHIZAIYAOQIUDEFANWEINEI,DANSHIPEICHUDEPEIYANGJIRUOBUFUHEYAOQIU,JIUYAOJINXINGBIYAODEDIAOZHENG。RUGUOYOUYIXIAOZHUNpHJI,KEYONGpHJI,RUGUOMEIYOU,KEYONGJINGMIDEpHSHIZHI,ZAIGENJUXUYAOYONG1 mol/LQINGYANGHUANAHUO1mol/LYANSUAN(WEIDIAOKEYONG0.1QINGYANGHUANAHUO0.1mol/LYANSUAN)DIAOZHISUOXUDEpH。PEIJIDEpHYIBANWEI7.4~7.6,YEYOUSUANXINGHUOJIANXINGDE。YONGQINGYANGHUANADIAOZHENGDEXUYAOGAOYAMIEJUNDEPEIYANGJI,ZAIDIAOZHENGpHSHIYAODIAOZHIGAOCHUSUOXU0.1GE~0.2GEDANWEI,YINYONGQINGYANGHUANADIAOZHENGSHI,GAOYAMIEJUNHOU,PEIYANGJIDEpHYAOJIANGDI0.1~0.2。RUPEIYANGJIZHONGHANYOUTANSUANGAICHENGFEN,KEBUpH。

 

 

SI、GUOLV

澳门老虎机PEICHENGDEPEIYANGJI,RUOWUTESHUYAOQIU,KESHENGLVECIBU。RUOYOUCHENDIANHUOHUNZHUO.XUYAOCHENGQINGDE.YETIPEIYANGJIKEYONGYOUZHIGUOLV。ERGUTIPEIYANGJIKEYONGZHONGJIANJIAYIBOCENGTUOZHIMIANDESHUANGCENGSHABUGUOLV。RUGUOGUOLVFAHAIBUNENGDADAOCHENGQINGDEYAOQIU,ZEKEYONGDANQINGCHENGQINGFA,JIPEIYANGJIJIARELENGQUEDAO50~60℃,ZHUANGRUSANJIAOPINGNEI(BUCHAOGUORONGLIANGDEERFENZHIYI),AN1000mLJIAREN1GE~2GEJIDANDEDANQING,YONGLIYAOHUANG3-5min,GAOYAZHENGQIMIEJUN121℃、20min,QUCHUCHENREGUOLVJIKE。

WU、FENZHUANG

PEIZHIHAODEPEIYANGJIGENJUBUTONGDEYONGTUFENZHUANGZAISANJIAOPING、SHIGUANDENGRONGQIZHONG,FENZHUANGSHIGUAN,RUGUOSHIGUANLIANGHENDA,KEYONGZIDONGFENYEQI,RUGUOSHIGUANLIANGSHAO,KEYONGLOUDOUFENZHUANG。FENZHUANGLIANGBUCHAOGUORONGQIRONGJIDESANFENZHIER。SANJIAOPINGYIBUCHAOGUORONGJIDEERFENZHIYIWEIYI;QIONGZHIXIEMIANYIBUCHAOGUOSHIGUANZHANGDUDEWUFENZHIYIWEIYI,MIEJUNHOU,BAICHENGDEXIEMIANWEIPEIYANGJILIANGDESANFENZHIYI,DICENGWEISANFENZHIERDELIANGWEIYI;BANGUTIQIONGZHIFENZHUANGLIANGWEISHIGUANZHANGDUDESANFENZHIYI;YONGLAIJIEZHONGHUOBAOJUNDEGAOCENGQIONGZHI,FENZHUANGLIANGWEISHIGUANZHANGDUDESIFENZHIYIHUOSANFENZHIYI,YONGLAIJIEZHONGYANYANGJUNDEYAODADAOSANFENZHIER;QIONGZHIPINGBAN,NEIJINGWEI90mmDEYI13mL~15 mLWEIYI,NEIJING70mmDEPINGBANWEI8mL~10 mL,ZHICHENGDEQIONGZHIPINGBANRUOBIAOMIANSHUIFENJIAODUO,KEJIANGPINGBANDAOKOU,FANGZHIZAI37℃PEIYANGXIANGNEI30min,GANZAOHOUZAIYONG。MEIPIPEIYANGJIYINGLINGWAIFENZHUANGYIXIAOBOLIPING(YUE20mL)YUGAIPIPEIYANGJITONGSHIMIEJUN,WEICEDINGGAIPIPEIYANGJIZHONGpH。

 

 

LIU、MIEJUN

FENZHUANGHAODEPEIYANGJIYINGLIJIJINXINGMIEJUN。MIEJUNDEFANGFAZHONGLEIRUXIA:

1.       GAOYAZHENGQIMIEJUNFA

DADUONAIREPEIYANGJIDOUKEYONGCIFA,XIAOLIANGFENZHUANGSHI,YONG121℃,15min;MIEJUNLIANGDASHI,YONG121℃,30minMIEJUN;HANTANGLEIDEPEIYANGJIZHINENG113~115℃,15minMIEJUN,BIMIANTANGLEIZAOPOHUAI。

2.ZHUFEIMIEJUNFA

DUIHANYOUBUNAIGAOWENWUZHIDEPEIYANGJI,KESHIYONGCIFANGFA。

3.GUOLVCHUJUN

YIGUOLVDEFANGFACHUJUN,YONGWUJUNCAOZUOJISHU,DINGLIANGJIARUPEIYANGJI。XUEYE、KANGSHENGSUKEYONGWUJUNCAOZUOJISHUCHOUQU,JIARULENGQUEDAO50℃ZUOYOUDEPEIYANGJIZHONG。PEIYANGJIHANYOUBUNAIREDEWUZHISHI,KEYONGCIFA。

DUILSTPEIYANGJIJINXINGMIEJUNSHI,YOUSHIFAJIAOGUANNEIHUIYOUQIPAO。WEIFANGZHIFAJIAOGUANNEICHANSHENGQIPAO,KEYICAIQUYIXIAJIXIANGCUOSHI:

1. XIAODAOGUANJINMANPEIYANGJI(BULIUQIPAO)HOUZAIJIARUDAOSHENGLSTDESHIGUANZHONG。

澳门老虎机2.MIEJUNGUOGUANBIFANGQIFAQIAN,JIANGGUONEIQITIPAIGANJING。

澳门老虎机3.SHIGUANSAIBUYAOSAIDETAIJIN(SHIYONGGUIJIAOSAIDESHIHOU),WUSHIYONGXIANGPISAI。

4BUYAOGUOZAODAKAIMIEJUNGUO,YAODENGMIEJUNGUONEIQIYAHEWENDUDOUJIANGDAOYUSHIWENYIZHIHUOXIANGCHABUDASHIZAIDAKAIMIEJUNGUO。

RUGUOYISHANGQINGKUANGDOUZUODAOHAIYOUQIPAO,KEYONGSHUIZUOPEIYANGJIZUDEDUIZHAOSHIYAN,RUOPEIYANGJIZUYOUQIPAO,ERDUIZHAOZUMEIYOUQIPAOKEQUEDINGSHIPEIYANGJIZISHENDEYUANYIN。

 

QI、DAOPINGBAN

澳门老虎机MIEJUNRONGHUADEPEIYANGJILENGQUEDAO50℃HOU,DAORUWUJUNGANZAODEPEIYANGMINZHONG。PEIYANGJIDEWENDUBUNENGGUOGAO,FOUZERONGYIZAIPEIYANGMINDENEIGAISHANGXINGCHENGTAIDUODELENGNINGSHUI;WENDUTAIDI,PEIYANGJIYOURONGYININGGUCHENGKUAI,WUFAZHICHENGPINGBAN。DAOPINGBANSHI,YINGZAIKAOJINJIUJINGDENGHUOYANJINXING,YIMIANWAIJIEDEZAJUNLUORUPINGBANNEI,ZUOSHOUNAPEIYANGMIN,YOUSHOUNASANJIAOPINGDEDIBU,YONGZUOSHOUDEXIAOZHIHESHOUZHANGBUWEIBASANJIAOPINGDEMIANSAIBAXIA,ZHUOSHAOPINGKOU,YONGDAMUZHIHESHIZHIBAPEIYANGMINGAIDAKAIYITIAOFENG,ZHIPINGKOUGANGHAOSHENRU,DAORUPEIYANGJI,YIPUMANDIWEIXIAN,BUCHAOGUOPEIYANGMINGAODUDESANFENZHIYI,XUNSUGAIHAOGAI,FANGZAIZHUOMIANSHANG,QINGQINGDIZHUANDONGPEIYANGMIN,SHIPEIYANGJIFENBUJUNYUN,LENGNINGHOUJIKE。

BA、BAIXIEMIAN

ZHUANGZAISHIGUANZHONGDEQIONGZHIPEIYANGJI,ZAIMIEJUNWANCHENGHOU,CHENRELIJIBAIFANGZAIMUBANG(HUOBOLIBANG)SHANG,BINGCHENGSHIDANGDEXIEDU,LENGQUEHOU,QIONGZHININGGUJICHENGXIEMIAN。XIEMIANZHANGDUBUYONGCHAOGUOSHIGUANERFENZHIYI。

JIU、ZHILIANGJIANCHA

PEIYANGJIMIEJUNHOUZIXIJIANCHAYIBIAN,FAXIANYOUPOLIE、SHUIFENJINRU、SEZEYICHANG、MIANSAIBEIPEIYANGJIZHANRAN,DOUYAODIUQI,BUNENGZAIYONG。BINGCEDINGQIZHONGpH。HAIXUJINXINGWUJUNJIANCHAHEXIAOGUOJIANCHA。WUJUNJIANCHASHIJIANGMIEJUNHOUDEPEIYANGJIQU1GUAN(PING)~2GUAN(PING),37℃HENGWENPEIYANG1d~2d,QUEDINGWUZAJUNSHENGZHANG;XIAOGUOJIANCHASHIYONGBIAOZHUNJUNZHUJIEZHONGZAIXIANGGUANDEPEIYANGJISHANGJIANCHAJIANCHAJUNDESHENGZHANG、XINGTAIJISHENGHUAQINGKUANG,YUYIZHIQINGKUANGXIANGFU。LIANGZHONGQINGKUANGDOUHEGE,PEIZHIDEPEIYANGJIFANGKESHIYONG。

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